Product Details

Isocove’s Modified Dulbecco’s Medium (IMDM) (1X), Liquid
With L-glutamine
With 25 mM HEPES
Without α-thioglycerol
Without 2-mercaptoethanol

Catalog Number LM 004-01
Storage Temperature 2~8°C

Product Description
In the late 1970s, Iscove and Guilbert demonstrated that the precursor cells of erythrocytes and macrophages could be cultured in low-serum medium supplemented with albumin, transferrin, lecithin and selenium. IMDM has a modified formulation of DMEM, containing additional amino acids and vitamins. Selenium, sodium pyruvate, and HEPES buffer are added. Fe(NO3)3·9H2O is substituted by KNO3 in this medium. Murine B lymphocyte, hemopoietic tissue derived from bone marrow, B lymphocyte activated by lipopolysaccharide, T lymphocyte, and a variety of hybrid cells are cultured in this medium.

LM 004-01 contains 584 mg/L L-glutamine and 25 mM of HEPES, but no α-thioglycerol and 2-mercaptoethanol. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Storage/Stability
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Biological Performance Characteristics
The growth-promoting capacities of IMDM are tested in a medium using an appropriate cell line. Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity. 

Precautions
For In Vitro Use Only

* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH. 

Comparison of liquid IMDM

Comparison of special ordered liquid IMDM

* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.